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Related Experiment Videos

Longevity of donor serum.

Jennifer E Graves1, H Lee Higdon, Jane E Johnson

  • 1Department of Obstetrics and Gynecology, Greenville Hospital System, South Carolina 29605-5601, USA.

Journal of Assisted Reproduction and Genetics
|June 10, 2004
PubMed
Summary

Long-term frozen serum remains viable for human in vitro fertilization. Stored serum up to 12 years supports embryo development, ensuring nutrient viability for assisted reproductive technologies.

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Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Cryobiology

Background:

  • Serum is a critical component in human in vitro fertilization (IVF) media, providing essential nutrients.
  • The long-term viability of stored serum is crucial for consistent and effective IVF outcomes.
  • Understanding serum stability over extended storage periods is vital for optimizing assisted reproductive technologies.

Purpose of the Study:

  • To assess the viability of long-term stored serum for use in human in vitro production.
  • To evaluate serum's efficacy as a source of energy substrates, amino acids, vitamins, and growth factors after prolonged cryopreservation.

Main Methods:

  • A prospective cohort study utilizing two-cell mouse embryos.
  • Serum samples stored for 1, 5, 10, and 12 years underwent four replications of mouse embryo testing.

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  • Embryo development was assessed by blastocyst rates.

    • Main Results:

    • No significant differences in blastocyst rates were observed between serum stored for different time intervals (1-12 years).
    • Blastocyst rates consistently ranged from 80% to 100% across all tested serum storage durations.
    • Pooled replicate means showed no statistically significant variation based on storage time.

    Conclusions:

    • Serum can be reliably frozen and stored for up to 12 years without compromising its developmental support capacity.
    • Long-term cryopreservation of serum is a viable option for human IVF.
    • This finding supports the continued use of stored serum in assisted reproductive technologies.