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Characterization of canine caspase-3
Junichi Sano1, Keisuke Oguma, Rui Kano
1Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
The Journal of Veterinary Medical Science
|June 10, 2004
Summary
Researchers cloned and sequenced the canine caspase-3 gene, finding significant homology with other species. Caspase-3 mRNA expression was confirmed in various healthy canine tissues.
Area of Science:
- Molecular Biology
- Genetics
- Comparative Genomics
Background:
- Caspase-3 is a key executioner caspase involved in apoptosis.
- Understanding canine caspase-3 provides insights into canine health and disease.
- Limited information exists on the canine caspase-3 gene sequence and expression.
Purpose of the Study:
- To clone and sequence the canine caspase-3 gene.
- To analyze the homology of canine caspase-3 with other species.
- To determine the tissue-specific expression of caspase-3 mRNA in dogs.
Main Methods:
- Canine genomic DNA was used for gene cloning and sequencing.
- Bioinformatic analysis was performed to predict amino acid sequence and homology.
- Reverse Transcription Polymerase Chain Reaction (RT-PCR) was used to analyze mRNA expression.
Main Results:
- The canine caspase-3 cDNA clone was 1473 base pairs, encoding 277 amino acids.
- High homology was observed between canine caspase-3 and human (88.4%), pig (88.0%), and mouse (85.9%).
- Caspase-3 mRNA was detected in healthy dog skin, lymph node, bone marrow, small intestine, and lung.
Conclusions:
- The canine caspase-3 gene has been successfully cloned and sequenced.
- Canine caspase-3 shares significant sequence homology with orthologs in other species.
- Caspase-3 is expressed in multiple tissues in healthy dogs, suggesting a vital role.