Related Experiment Video
Updated: Aug 24, 2026

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass
Published on: May 27, 2022
Quantitative analysis of cannabinoids from Cannabis sativa using 1H-NMR
Arno Hazekamp1, Young Hae Choi, Robert Verpoorte
1Division of Pharmacognosy, Institute of Biology, Leiden University, Einsteinweb 55, 2300 RA Leiden, The Netherlands. ahazekamp@rocketmail.com
Abstract:
A (1)H-NMR method has been developed for the quantitative analysis of pure cannabinoids and for cannabinoids present in Cannabis sativa plant material without any chromatographic purification. The experiment was performed by the analysis of singlets in the range of delta 4.0-7.0 in the (1)H-NMR spectrum, in which distinguishable signals of each cannabinoid are shown. Quantitation was performed by calculating the relative ratio of the peak area of selected proton signals of the target compounds to the known amount of the internal standard, anthracene. For this method no reference compounds are needed. It allows rapid and simple quantitation of cannabinoids with a final analysis time of only 5 min without the need for a pre-purification step.
Related Concept Videos
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
NMR Spectroscopy of Aromatic Compounds
¹H NMR Signal Integration: Overview
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

