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Updated: Aug 24, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Interferences in clinical capillary zone electrophoresis of serum proteins
1Laboratory Medicine/Immunology, University Hospitals Leuven, Herestraat 49, B-3000 Leuven, Belgium. xavier.bossuyt@uz.kuleuven.ac.be
Abstract:
Capillary zone electrophoresis (CZE) of serum proteins has become a well-accepted method for the separation of serum proteins and for the detection of monoclonal proteins in clinical laboratories. As CZE uses ultraviolet detection for direct protein quantification, exogenous ultraviolet-absorbing substances are a novel challenge, not present in conventional gel-based methods in which protein is quantified by dye binding. This minireview gives a survey of interfering substances, including iodinated contrast agents and antibiotics.
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