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Related Experiment Videos

A proteome reference map for Vibrio cholerae El Tor.

Ana Coelho1, Eidy de Oliveira Santos, Mauro Luiz da Hora Faria

  • 1Proteomics Network of Rio de Janeiro-FAPERJ, and Department of Genetics, Institute of Bilogia, Universidade Federal do Rio de Janeiro, Brazil. coehlo@biologia.ufrj.br

Proteomics
|June 10, 2004
PubMed
Summary

This study maps the Vibrio cholerae El Tor proteome using 2D gels, identifying 80 abundant proteins and confirming hypothetical proteins. It reveals a significant abundance of periplasmic ATP-binding cassette transporter proteins.

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Area of Science:

  • Microbiology
  • Proteomics
  • Molecular Biology

Background:

  • Vibrio cholerae El Tor is a significant human pathogen.
  • Understanding its proteome is crucial for developing effective interventions.
  • Previous proteomic studies have limitations in scope and depth.

Purpose of the Study:

  • To construct a comprehensive proteome reference map for Vibrio cholerae El Tor.
  • To identify abundant proteins and experimentally confirm hypothetical proteins.
  • To compare proteomic profiles between different strains and growth conditions.

Main Methods:

  • Two-dimensional gel electrophoresis (2-D gels) with immobilized pH gradients.
  • Peptide mass fingerprinting and mass spectrometry for protein identification.

Related Experiment Videos

  • Comparative analysis of two Vibrio cholerae El Tor strains (N16961 and C3294) under different media conditions.

    • Main Results:

    • A consensus proteome map comprising 340 spots was generated.
    • 80 abundant proteins were identified, including energy metabolism components and periplasmic ATP-binding cassette (ABC) transporter proteins.
    • Two OmpU isoforms were detected, and seven hypothetical proteins were experimentally confirmed.

    Conclusions:

    • The study provides a detailed proteome map for Vibrio cholerae El Tor.
    • It highlights the significant abundance of periplasmic ABC transporter proteins, a finding not evident in prior microarray studies.
    • The confirmed hypothetical proteins offer new targets for further research into V. cholerae pathogenesis.