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Updated: Aug 24, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Cloning and identification of NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural
Qian Yang1, Jun Cheng, Yan Liu
1Department of Infectious Diseases, First Hospital of Xi'an Jiaotong University, Shaanxi Province, China.
Aim:
To clone, identify and study new NS5ATP2 gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A.
Methods:
On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify NS5ATP2 gene.
Results:
The coding sequence of a new gene and its spliced variant were cloned and identified successfully.
Conclusion:
A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins.
