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[Inhibitory effect of hDAF displayed on yeast cells on deposition of complement on the yeast cells]
Bo Guo1, Ping Zheng, Pei-rong Xie
1Department of Immunology, Third Military Medical University, Chongqing 400038, China.
Aim:
To investigate whether the hDAF displayed on the surface of yeast cells can inhibit the deposition of C5b-9.
Methods:
After treatment with normal human serum, deposition of human complement C5b-9 and DAF expression on yeast cells EBY100 were analyzed by flow cytometry.
Results:
Yeast cells EBY100 could activate the complement molecules in human serum and lead to the deposition of C5b-9, while the deposition of C5b-9 on the surface of yeast cells with hDAF could significantly reduce the deposition of C5b-9.
Conclusion:
hDAF displayed on the surface of yeast cells can fold correctly and exhibit activity of complement's inhibition.
