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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
Inhibition of virus production in JC virus-infected cells by postinfection RNA interference
Yasuko Orba1, Hirofumi Sawa, Hiroshi Iwata
1Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Kita-ku, Sapporo 060-8638, Japan.
Abstract:
RNA interference has been applied for the prevention of virus infections in mammalian cells but has not succeeded in eliminating infections from already infected cells. We now show that the transfection of JC virus-infected SVG-A human glial cells with small interfering RNAs that target late viral proteins, including agnoprotein and VP1, results in a marked inhibition both of viral protein expression and of virus production. RNA interference directed against JC virus genes may thus provide a basis for the development of new strategies to control infections with this polyomavirus.
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