Related Experiment Video

Updated: Aug 23, 2026

Pseudomonas aeruginosa Induced Lung Injury Model
07:24

Pseudomonas aeruginosa Induced Lung Injury Model

Published on: October 29, 2014

PMN apoptosis and its relationship with the lung injury after chest impact trauma

Ren Liu1, Shuang-ding Li, Jia-xin Min

  • 1Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. liu0916@mail.china.com

Chinese Medical Journal
|June 17, 2004
PubMed
Abstract

Insights

Delayed polymorphonuclear neutrophil (PMN) apoptosis after chest trauma contributes to lung injury. This PMN dysfunction, potentially driven by increased intracellular calcium, exacerbates inflammation and tissue damage.

Area of Science:

  • Immunology
  • Trauma Research
  • Cell Biology

Background:

  • Polymorphonuclear neutrophils (PMNs) are key inflammatory cells involved in all phases of inflammation.
  • Chest impact trauma can lead to significant inflammatory responses and tissue damage.

Purpose of the Study:

  • To investigate the relationship between PMN apoptosis and lung injury following chest impact trauma.
  • To understand the role of PMN behavior in the inflammatory process post-trauma.

Main Methods:

  • PMNs were isolated from rabbits experiencing chest impact trauma.
  • Apoptosis, necrosis, survival, and respiratory burst of PMNs were analyzed using flow cytometry.
  • Lactate dehydrogenase (LDH) levels and intracellular calcium ([Ca2+]i) were measured.

Main Results:

  • Delayed PMN apoptosis and increased viable cells were observed between 2 and 12 hours post-trauma.
  • PMN respiratory burst activity significantly increased from 2 hours, peaking at 8 hours.
  • Elevated LDH and transient increases in intracellular free Ca2+ in PMNs were noted post-trauma.

Conclusions:

  • Impaired PMN apoptosis and retention in tissues lead to sustained PMN activation and release of damaging substances.
  • The observed increase in intracellular free Ca2+ may be a key factor in the delayed PMN apoptosis.
  • These findings highlight PMN apoptosis as a critical determinant of lung injury severity after chest trauma.

More Related Videos

Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs08:58

Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs

Published on: October 31, 2025

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury14:48

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Published on: March 21, 2021

Related Concept Videos

Flail Chest-II01:26

Flail Chest-II

Managing flail chest, a condition characterized by a segment of the chest wall moving independently from the rest of the thoracic cage, requires a comprehensive approach. It includes a thorough assessment of the patient's condition, a diagnostic evaluation to determine the extent of the injury, and the implementation of appropriate medical interventions tailored to the individual's needs.
Assessment:
1. Clinical Evaluation:
History:
Pneumothorax II: Pathophysiology01:08

Pneumothorax II: Pathophysiology

Pneumothorax means the presence of air in the pleural space — the thin potential gap between the visceral and parietal pleura. This condition disrupts the normal pressure balance that keeps the lungs inflated, leading to partial or complete collapse of the affected lung.Normal physiologyUnder normal conditions, the pleural space maintains a slightly negative intrapleural pressure, which keeps the lungs expanded against the chest wall. This negative pressure creates a delicate balance between...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
Pneumothorax-I01:26

Pneumothorax-I

A pneumothorax is a condition where air builds up in the space between the lung and the chest wall, causing the lung to collapse. This condition arises when air enters the space between the parietal and visceral pleura, disrupting the negative pressure essential for lung inflation. This can lead to a partial or complete collapse of the lung.
Pneumothorax can be even further classified as spontaneous, traumatic, and tension pneumothorax.
Cellular Injury IlI: Cellular Death01:11

Cellular Injury IlI: Cellular Death

Cell death is the irreversible loss of cellular structure and function, representing the final stage of severe injury. It plays a key role in both normal physiology and disease.Types of Cell DeathThe two main types are necrosis and apoptosis, though others like necroptosis and pyroptosis also exist.Necrosis:Necrosis is an unregulated form of cell death caused by severe injury such as trauma, toxins, or ischemia. It is characterized by cell swelling, membrane loss, rupture, and leakage of...