Related Experiment Video

Updated: Aug 23, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
07:52

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Published on: September 10, 2018

Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

Taek Jin Kang1, Hui Kyoung Song, Jin Ho Ahn

  • 1School of Chemical Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea.

Biotechnology Letters
|June 18, 2004
PubMed

More Related Videos

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells08:28

Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

Published on: May 9, 2025

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA07:13

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Published on: April 26, 2011

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.