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Published on: November 13, 2014
Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus
Elizabeta Hernández-Domínguez1, Freddy Campos-Tamayo, Felipe Vázquez-Flota
1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacíon Cientifíca de Yucatán, Calle 43 No. 130 Chuburná 97200, Mérida Yucatán, México.
Abstract:
Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g(-1) dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d.
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