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Updated: Aug 23, 2026

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

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Published on: May 14, 2016

[Apoptin induces G2-M arrest in cancer cells]

Xiang-jun He1, Shi-tong Shang, Yu-jing Liu

  • 1Central Laboratory, Peking University People's Hospital, Beijing 100044, China. hexiangjun@hotmail.com

Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences
|June 19, 2004
PubMed
Abstract

Insights

Apoptin effectively induces cancer cell apoptosis by causing chromatin condensation and G2-M cell cycle arrest, showing promise for targeted cancer therapy.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Virology

Context:

  • Apoptin is a protein with demonstrated tumor-specific activity.
  • Understanding its mechanism is crucial for developing novel cancer therapies.
  • Adenovirus vectors offer efficient delivery for gene-based treatments.

Purpose:

  • To investigate the mechanism of apoptin-induced cancer cell apoptosis.
  • To evaluate the application of apoptin in cancer therapy using recombinant adenoviruses.

Summary:

  • Apoptin gene was cloned into an adenovirus vector (Ad/apoptin).
  • Cancer cells infected with Ad/apoptin exhibited nuclear localization of apoptin.
  • Morphological changes, including chromatin condensation and G2-M cell cycle arrest, were observed.

Impact:

  • Apoptin induces cancer cell-specific apoptosis through cell cycle arrest.
  • This study supports the potential of apoptin as a targeted cancer therapeutic agent.
  • Further research can explore optimizing Ad/apoptin delivery and efficacy.

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