Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Candida parapsilosis characterization in an outbreak setting.

Duncan M Kuhn1, Pranab K Mikherjee, Thomas A Clark

  • 1University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 44106, USA.

Emerging Infectious Diseases
|June 23, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving Growth Dynamics of <i>Faecalibacterium prausnitzii</i> by Exposure to Prebiotics.

International journal of molecular sciences·2026
Same author

Reply to Itagaki et al. Comment on "Retuerto et al. Analysis of Gut Bacterial and Fungal Microbiota in Children with Autism Spectrum Disorder and Their Non-Autistic Siblings. <i>Nutrients</i> 2024, <i>16</i>, 3004".

Nutrients·2025
Same author

Bad to the bone: <i>Candida (Candidozyma) auris</i> vertebral osteomyelitis treated with combination antifungal therapy followed by a novel long-acting echinocandin.

ASM case reports·2025
Same author

Transglutaminase 2 in chronic inflammation and fibrosis - a potential novel therapy for stricturing Crohn's disease.

Expert review of clinical immunology·2025
Same author

The functional role of the extracellular matrix in inflammatory bowel disease associated gut fibrosis.

Matrix biology : journal of the International Society for Matrix Biology·2025
Same author

Immunomodulatory Properties of Multi-Strain Postbiotics on Human CD14<sup>+</sup> Monocytes.

Life (Basel, Switzerland)·2025

Candida parapsilosis outbreaks in hospitals are linked to specific genetic strains. These strains show increased biofilm production, suggesting it plays a key role in infections.

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Candida parapsilosis is a significant non-albicans Candida species causing hospital-acquired infections.
  • Limited research exists correlating C. parapsilosis outbreaks with specific virulence factors.

Purpose of the Study:

  • To investigate the genetic variability of C. parapsilosis isolates from an outbreak.
  • To correlate genotypic data with phenotypic virulence factors in C. parapsilosis.

Main Methods:

  • DNA fingerprinting was employed to assess genetic diversity among outbreak and clinical isolates.
  • Phenotypic virulence factors including adherence, biofilm formation, secretory aspartic protease (SAP), and phospholipase activity were quantified.

Main Results:

Related Experiment Videos

  • DNA fingerprinting revealed outbreak isolates were clonal and genetically distinct from other clinical isolates.
  • The outbreak clone exhibited significantly higher biofilm production compared to sporadic isolates (p < 0.0005).
  • Adherence and protein secretion levels did not correlate with strain pathogenicity.

Conclusions:

  • Biofilm production is a critical virulence factor implicated in Candida parapsilosis outbreaks.
  • The findings highlight the importance of biofilm formation in the pathogenesis of C. parapsilosis hospital infections.