Dynamics of insertion sequence elements during experimental evolution of bacteria
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Updated: Aug 9, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Dominique Schneider1, Richard E Lenski
1Laboratoire Adaptation et Pathogénie des Microorganismes, CNRS UMR5163, Université Joseph Fourier, 38041 Grenoble Cedex 9, France. dominique.schneider@ujf-grenoble.fr
Abstract:
We review the intersection between two areas of microbial evolution that were research foci of Michel Blot. One focus is the behavior of insertion sequence (IS) elements, including their role in promoting the evolutionary adaptation of their hosts. The other focus is experimental evolution, an approach that allows the dynamics of genomic and phenotypic change to be observed in the laboratory. This review shows that IS elements are useful as markers for detecting genomic change over experimental time scales and, moreover, that IS elements generate some of the beneficial mutations that increase organismal fitness.
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