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Updated: Aug 23, 2026
Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
[Isolation and characterization of Rahnella aquatilis lipopolysaccharides]
L D Varbanets1, A N Ostapchuk, N V Vinarskaia
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
Lipopolysaccharides (LPS) of six strains of Rahnella aquatilis isolated from freshwater reservoirs, plant rhizosphere, and from patient have been investigated. It was shown, that LPS of type strain was characterized by the presence of rhamnose and mannose as predominant monosaccharides, while LPS of the rest strains contained galactose and fucose. The glucosamine content was insignificant (0.42-1.0%) in LPS of all strains studied. It was shown by double immunodiffusion in agar, immunoelectrophoresis and rocket immunoelectrophoresis that all R. aquatilis LPS were antigens in homological systems. The results of serological cross reactions evidence for immunochemical heterogeneity of R. aquatilis species. LPS of R. aquatilis display pyrogeneity and toxicity (LD50 from 0.20 to 0.33 mg/kg of experimental animals weight).
