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Basic fibroblast growth factor-like immunoreactivity in the trigeminal proprioceptive and motor systems
Brain Research
|April 10, 1992
Summary
Basic fibroblast growth factor (bFGF) is transported within adult brain neurons, functioning in mature circuits. This study reveals bFGF is produced in ribosomes and endoplasmic reticulum, then moves into the nucleus.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Basic fibroblast growth factor (bFGF) is known to stimulate cell division, angiogenesis, and neuronal survival.
- Its transport mechanisms and subcellular localization in the adult brain remain incompletely understood.
Purpose of the Study:
- To investigate the transport and subcellular localization of endogenous bFGF in adult brain neurons.
- To elucidate the role of bFGF in mature neuronal circuits, specifically the masseteric reflex arc.
Main Methods:
- Immunohistochemistry was used to detect bFGF immunoreactivity in neurons of the mesencephalic nucleus of the trigeminal nerve.
- Electron microscopy was employed to examine the subcellular localization of bFGF, including its presence in ribosomes, endoplasmic reticulum, and nuclear compartments.
Main Results:
- Neurons in the mesencephalic trigeminal nucleus containing bFGF were identified, with projections to muscle spindles and the trigeminal motor nucleus.
- bFGF immunoreactivity was found in free ribosomes and rough endoplasmic reticulum, and also within the euchromatin of the nucleus.
- Axons containing bFGF formed synapses with motoneurons, suggesting transport within nerve processes.
Conclusions:
- Endogenous bFGF is transported within nerve fibers and actively participates in mature neuronal circuits, such as the masseteric reflex.
- bFGF is synthesized in free ribosomes and/or rough endoplasmic reticulum and subsequently transported into the genetically active euchromatin of the nucleus.