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Related Experiment Videos

S100 and cytokine expression in caries.

Julia L McLachlan1, Alastair J Sloan, Anthony J Smith

  • 1Oral Biology, School of Dentistry, The University of Birmingham, Birmingham B4 6NN, United Kingdom.

Infection and Immunity
|June 24, 2004
PubMed
Summary

This study reveals that chronic carious infections significantly increase molecular immune responses in dental pulp, with higher gene expression correlating to disease severity. Neutrophils are key players in this inflammatory process.

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Area of Science:

  • Immunology
  • Oral Biology
  • Molecular Biology

Background:

  • The molecular immune response in dental pulp during chronic caries is not well understood.
  • Understanding these responses is crucial for managing carious infections.

Purpose of the Study:

  • To investigate the expression of molecular mediators of pulpal inflammation in carious teeth.
  • To correlate these molecular levels with carious disease severity.
  • To determine the cellular localization of key inflammatory molecules.

Main Methods:

  • Semiquantitative reverse transcriptase PCR was used to analyze gene expression in 10 carious and 10 healthy pulpal tissue samples.
  • Expression levels of S100 family members, cytokines (TNF-alpha, IL-1beta, IL-8, IL-6, ENA-78), and collagen-1alpha were assessed.

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  • Immunohistochemistry was employed to identify the cellular localization of S100A8 and the S100A8/S100A9 complex.

    • Main Results:

    • Gene expression of S100A8, S100A9, S100A12, S100A13, TNF-alpha, IL-1beta, IL-8, IL-6, ENA-78, and collagen-1alpha was significantly higher in carious pulp compared to healthy pulp.
    • Increased gene expression correlated positively with carious disease severity.
    • S100A8 and the S100A8/S100A9 complex were primarily found in infiltrating neutrophils within carious pulpal tissue.

    Conclusions:

    • Chronic carious infection triggers a complex molecular immune response in dental pulp.
    • Neutrophils play a significant role, with their activation leading to the upregulation of specific S100 proteins.
    • The findings provide insights into the inflammatory mechanisms underlying carious lesions.