Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Amphotericin B channel conductance inactivation].

V Kh Ibragimova1, I N Alieva, D I Aliev

  • 1Institute of Radiation Problems, National Academy of Sciences of Azerbaijan Republic, Baku.

Tsitologiia
|June 26, 2004
PubMed
Summary

Amphotericin B forms transient pores in cholesterol-containing membranes, transitioning between nonconducting and conducting states. This biphasic kinetics is cholesterol-dependent, unlike ergosterol membranes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Long-term treatment of morphologically verified myocarditis: successes and probable errors. Case report].

Terapevticheskii arkhiv·2023
Same author

[Multislice spiral computed tomography of the heart in dilated cardiomyopathy: possibilities in the verification of myocarditis (in comparison with myocardial biopsy) and in the evaluation of prognosis].

Terapevticheskii arkhiv·2018
Same author

[The spatial organization and conformational flexibility of neuropeptides of the gallatostatin family].

Biofizika·2005
Same author

[Conformational properties of neuromedin NmU-8 and its modified analogs].

Molekuliarnaia biologiia·2004
Same author

[Radioprotective and antineoplastic activity of polyene antibiotics combined with dimethyl sulfoxide].

Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]·2003
Same author

[Biophysical and medical and biological aspects of use of polyene antibiotics in combination with dimethylsulfoxide].

Biofizika·2002

Area of Science:

  • Membrane biophysics
  • Antifungal drug mechanisms
  • Lipid-protein interactions

Context:

  • Amphotericin B is a vital antifungal agent that interacts with cell membranes.
  • Its mechanism of action involves pore formation, but the kinetics are complex.
  • Cholesterol is a key component of mammalian cell membranes, influencing drug interactions.

Purpose:

  • To investigate the effects of Amphotericin B and its derivatives on bilayer lipid membranes.
  • To elucidate the kinetics of membrane conductance changes induced by the antibiotic.
  • To understand the role of cholesterol in Amphotericin B-induced membrane alterations.

Summary:

  • Amphotericin B induces transient, multichannel membrane conductance in a concentration-dependent manner.
  • Biphasic kinetics suggest a multi-step pore formation and disassembly process involving nonconducting and conducting states.
  • Cholesterol-containing membranes exhibit this biphasic behavior, while ergosterol-containing membranes show monotonic kinetics.

Impact:

  • Reveals a dynamic model for polyene channel formation and dissociation within lipid bilayers.
  • Highlights the critical role of membrane cholesterol in mediating Amphotericin B's activity.
  • Provides insights into the molecular basis of Amphotericin B's antifungal efficacy and potential side effects.

Related Experiment Videos