Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bioengineered teeth from cultured rat tooth bud cells.

M T Duailibi1, S E Duailibi, C S Young

  • 1University Federal of São Paulo, Department of Otorhinolaryngology and Human Communication Disorders, Sao Paolo, Brazil.

Journal of Dental Research
|June 26, 2004
PubMed
Summary

Researchers improved tooth bioengineering by using cultured rat tooth bud cells. Four-day post-natal rat cells on scaffolds reliably generated bioengineered tooth tissues, showing potential for dental regeneration.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing a Global Oral Health Research Agenda.

Journal of dental research·2026
Same author

Tooth-on-a-chip to engineer early dental epithelial-mesenchymal interaction.

Materials today. Bio·2025
Same author

Reimagining Dental, Oral, and Craniofacial Research: New Opportunities for the IADR's Efforts to Achieve Global Oral Health.

Journal of dental research·2025
Same author

Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

Physical review letters·2024
Same author

Lawson Criterion for Ignition Exceeded in an Inertial Fusion Experiment.

Physical review letters·2022
Same author

Thrombotic thrombocytopenia following ChAdOx1 nCov-19 vaccination.

Acute medicine·2021

Area of Science:

  • Bioengineering
  • Regenerative Medicine
  • Dental Tissue Engineering

Background:

  • Mammalian dental tissue regeneration shows promise through bioengineering complex tooth structures from pig tooth bud tissues.
  • Optimizing methods for bioengineering dental tissues is crucial for advancing regenerative medicine.

Purpose of the Study:

  • To improve tooth bioengineering methods by evaluating cultured rat tooth bud cells from different post-natal days (3-7 dpn).
  • To determine the optimal cell source and seeding conditions for reliable bioengineered tooth tissue generation.

Main Methods:

  • Cultured rat tooth bud cells from 3- to 7-day post-natal rats were seeded onto polyglycolic acid (PGA) or polylactic-co-glycolic acid (PLGA) scaffolds.
  • Scaffolds were implanted into the omenta of adult rat hosts for 12 weeks.

Related Experiment Videos

  • Implanted tissues were harvested and analyzed to assess bioengineered tooth tissue formation.

    • Main Results:

    • Dissociated 4-day post-natal (dpn) rat tooth bud cells, when seeded for 1 hour onto PGA or PLGA scaffolds, most reliably generated bioengineered tooth tissues.
    • Analysis of 12-week implants confirmed successful generation of tooth tissues from the optimized cell source and seeding conditions.
    • The study demonstrated successful bioengineering of both pig and rat tooth tissues.

    Conclusions:

    • Tooth tissue engineering methods are effective for generating dental tissues in both pig and rat models.
    • The successful bioengineering of tooth structures from cultured tooth bud cells indicates that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.
    • Four-day post-natal rat tooth bud cells represent a promising source for regenerative dental applications.