Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional analysis of APC-Cdh1.

Tamotsu Sudo1, Naoto T Ueno, Hideyuki Saya

  • 1Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|June 29, 2004
PubMed
Summary

Cell cycle checkpoints maintain genomic integrity. The Cdh1-APC complex is crucial for G1 and G2 cell cycle arrests, making it a potential target for cancer therapeutics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Post-transcriptional regulation of mRNA stability in pancreatic ductal adenocarcinoma.

Frontiers in immunology·2026
Same author

Identification of <i>BRCA2</i> Likely Germline Pathogenic Variants in Patients with Multiple Primary Lung Adenocarcinomas.

Oncology research·2026
Same author

Proof-of-Concept of a DNA-Based Recording System for High-Throughput Functional Gene Screening.

ACS synthetic biology·2026
Same author

Genomic Alteration Patterns Across Histological Grades in BRAF p.V600E-Mutant Gliomas and Glioneuronal Tumors: An Analysis of 15 Cases.

Pathology international·2026
Same author

Genomic Profiling in Localized Prostate Cancer: Associations With Biochemical Recurrence and Response to Salvage Radiotherapy.

Cancer science·2026
Same author

Depleting HIF‑1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF‑independent pathways.

Oncology reports·2026

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell cycle checkpoints are essential for maintaining genomic stability during cell division.
  • Defective checkpoints lead to genomic aberrations and cancer development.
  • The anaphase-promoting complex (APC), regulated by Cdc20 and Cdh1, controls cell cycle progression.

Purpose of the Study:

  • To investigate the role of Cdh1-APC in regulating cell cycle checkpoints.
  • To explore the potential of cell cycle checkpoints as therapeutic targets in cancer.

Main Methods:

  • Analysis of three key cell cycle checkpoints: spindle assembly, rapamycin-induced G1, and DNA damage-induced G2.
  • Utilizing cell synchronization techniques for detailed checkpoint analysis.
  • Investigating the impact of Cdh1 deficiency on checkpoint function and cell cycle progression.

Main Results:

  • Cdh1-APC activity is critical for timely degradation of mitotic cyclins, ensuring proper progression from mitosis to G1.
  • Loss of Cdh1 function abrogates rapamycin-induced G1 arrest and impairs DNA damage-induced G2 arrest.
  • Cdh1-APC activation is induced by DNA damage, highlighting its role in DNA damage response.

Conclusions:

  • Activation of Cdh1-APC is vital for both cyclin-dependent kinase inhibitor-dependent G1 arrest and DNA damage-induced G2 arrest.
  • Cell cycle checkpoints, particularly those involving Cdh1-APC, represent promising targets for novel cancer therapies.
  • Understanding checkpoint mechanisms provides insights into cancer development and potential treatment strategies.

Related Experiment Videos