Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Post-proteasomal antigen processing for major histocompatibility complex class I presentation.

Kenneth L Rock1, Ian A York, Alfred L Goldberg

  • 1Department of Pathology, University of Massachusetts Medical Center, Worcester, MA 01655, USA.

Nature Immunology
|June 30, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A broad cathepsin inhibitor blocks crystal-stimulated inflammasome-dependent and -independent inflammation and gout arthritis.

Communications medicine·2026
Same author

Author Correction: Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization.

Nature immunology·2026
Same author

Pre-existing cross-reactive immunity to highly pathogenic avian influenza 2.3.4.4b A(H5N1) virus in the United States.

Nature communications·2025
Same author

Effect of Seasonal Influenza Vaccines on Avian Influenza A(H5N1) Clade 2.3.4.4b Virus Infection in Ferrets.

Emerging infectious diseases·2025
Same author

Epitope-Specific Antibody Immunodominance Driving Antibody and Influenza Viral Evolution During 2010- 2024.

Open forum infectious diseases·2025
Same author

Trained ILCs confer adaptive immunity-independent protection against influenza.

Journal of virology·2025
Same journal

Editorial Expression of Concern: Recognition of the nonclassical MHC class I molecule H2-M3 by the receptor Ly49A regulates the licensing and activation of NK cells.

Nature immunology·2026
Same journal

Inflammatory immune modulators of AML lung infiltration and respiratory failure.

Nature immunology·2026
Same journal

The neuroimmune system and cognition.

Nature immunology·2026
Same journal

Critical connections.

Nature immunology·2026
Same journal

Innate immune signaling and functions in astrocytes.

Nature immunology·2026
Same journal

Epigenetic mechanisms of inflammatory memory in the central nervous system.

Nature immunology·2026
See all related articles

Major histocompatibility complex class I (MHC) molecules present peptides derived from intracellular proteins. Proteasomes and peptidases control antigen presentation by generating or degrading these peptides.

Area of Science:

  • Immunology
  • Molecular Biology
  • Proteolysis

Background:

  • Major histocompatibility complex class I (MHC) molecules present peptides to T cells, crucial for immune surveillance.
  • These peptides originate from intracellular proteins degraded by proteasomes into oligopeptides.
  • Proteasomal degradation often yields N-extended peptide precursors, not mature epitopes.

Purpose of the Study:

  • To elucidate the proteolytic mechanisms governing the generation and processing of peptides for MHC class I presentation.
  • To understand the roles of proteasomes and various peptidases in shaping the antigenic peptide repertoire.

Main Methods:

  • Analysis of peptide generation by proteasomes.
  • Investigation of aminopeptidase and endopeptidase activities in peptide trimming and degradation.

Related Experiment Videos

  • Assessment of the balance between peptide synthesis and degradation.

    • Main Results:

    • Proteasomes generate C-terminal peptide precursors, often longer than required for MHC I binding.
    • Aminopeptidases in the cytosol and endoplasmic reticulum trim these precursors to form mature N-termini.
    • Endopeptidases, like thimet oligopeptidase, and aminopeptidases can degrade peptides, limiting epitope supply.

    Conclusions:

    • Antigen presentation is tightly regulated by a balance between proteolytic processes that generate and degrade peptides.
    • The interplay between proteasomes and peptidases determines the availability of antigenic peptides for MHC class I presentation.
    • Understanding these proteolytic pathways is key to modulating immune responses.