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Updated: Aug 23, 2026

A Method of Nodose Ganglia Injection in Sprague-Dawley Rat
Published on: November 25, 2014
Distribution of vanilloid receptors in the rat laryngeal innervation
Shinobu Koike1, Toshiyuki Uno, Hitoshi Bamba
1Department of Otolaryngology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Objective:
Capsaicin is known to selectively activate nociceptic sensory neurons through vanilloid receptors. In this study we investigated the distribution of vanilloid receptor subtype 1 (VR1) and vanilloid receptor-like protein 1 (VRL-1) in the rat larynx.
Material And Methods:
The distributions of VR1 and VRL-1 were determined immunohistochemically. The colocalization of vanilloid receptors with common choline acetyltransferase (cChAT), vasoactive intestinal polypeptide (VIP), substance P (SP) and neuronal nitric oxide synthase (nNOS) was also studied using an immunohistochemical double-labeling technique.
Results:
VRL-1-positive fibers were detected in the laryngeal epithelium and lamina propria. VR1-positive nerve fibers were seen in the lamina propria but not in the mucosal epithelium. VR1- and VRL-1-positive cells were distributed in the intralaryngeal ganglia and colocalization of capsaicin receptors with VIP, nNOS and cChAT was seen.
Conclusion:
These findings suggest that these capsaicin receptors participate in the parasympathetic innervation as well as in nociception of the rat larynx.
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