Related Experiment Video

Updated: Aug 23, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Cell type-specific regulation of calmodulin 2 expression by mutant p53

K X Knaup1, K Roemer

  • 1Department of Virology, University of Saarland Medical School, Bldg. 47, 66421 Homburg/Saar, Germany. karl.knaup@uniklinik-saarland.de

FEBS Letters
|July 1, 2004
PubMed

Insights

Oncogenic mutant p53 (175H) specifically stimulates calmodulin 2 gene expression in fibroblasts. This requires promoter sequences and the mutant p53 transactivation domain, but not direct binding.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Mutant p53 proteins can acquire oncogenic functions.
  • Understanding gene expression changes induced by mutant p53 is crucial for cancer therapy.

Purpose of the Study:

  • To identify genes upregulated by oncogenic mutant p53 (175H).
  • To investigate the mechanism of mutant p53-mediated gene stimulation.

Main Methods:

  • Microarray analysis and PCR-select subtractive hybridization were used to profile gene expression.
  • Human wild-type p53-negative immortal 041 fibroblasts were stably infected to express mutant p53 175H.
  • Reporter assays and in vitro binding assays were employed to study promoter activity and protein-DNA interactions.

Main Results:

  • Mutant p53 175H induced limited gene expression changes compared to wild-type p53.
  • Calmodulin 2 (CaM 2) gene expression was specifically stimulated by mutant p53 175H in 041 cells.
  • CaM 2 promoter stimulation by 175H required its 5' untranslated sequences and transactivation domain integrity.
  • Direct in vitro binding of 175H to the 5' untranslated region was not detected.

Conclusions:

  • Oncogenic mutant p53 175H can specifically induce calmodulin 2 gene expression.
  • The mechanism involves promoter regulatory elements and the mutant p53 transactivation domain, suggesting indirect DNA interaction.
  • These findings contribute to understanding the transcriptional targets and mechanisms of oncogenic mutant p53.

More Related Videos

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...