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Updated: Aug 23, 2026
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Intrinsic factor-mediated modulation of cyanocobalamin-N-sulfonyl-acridinium-9-carboxamide chemiluminescence
Maciej Adamczyk1, Donald D Johnson, Phillip G Mattingly
1Department of Chemistry (D09MD), Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, AP20, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Abstract:
Two cyanocobalamin-N-sulfonyl-acridinium-9-carboxamides with linkage through the N(10) or 9-position were prepared from B(12)-e-carboxylic acid. The noncovalent association of intrinsic factor with these ligands resulted in specific modulation of the associated chemiluminescence signal either by quenching or changing the emission profile. Either effect was sufficient to formulate a homogeneous assay to detect vitamin B(12) in buffer.
