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Related Experiment Videos

CD133, a novel marker for human prostatic epithelial stem cells.

Gavin D Richardson1, Craig N Robson, Shona H Lang

  • 1Prostate Research Group, Surgical Oncology, Medical School, Framlington Place, University of Newcastle upon Tyne, Newcastle upon Tyne, NE2 4HH, UK.

Journal of Cell Science
|July 1, 2004
PubMed
Summary

Researchers identified a specific marker for prostate stem cells. These CD133-positive cells have self-renewal and differentiation properties, crucial for understanding prostate cancer and benign prostatic hyperplasia.

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Area of Science:

  • Urology
  • Stem Cell Biology
  • Cancer Research

Background:

  • Prostate stem cells are critical for tissue homeostasis and regeneration.
  • Identifying specific markers for prostate stem cells is essential for understanding their role in diseases like prostate cancer and benign prostatic hyperplasia.
  • Current characterization of stem cells is limited due to the lack of definitive molecular markers distinguishing them from progeny cells.

Purpose of the Study:

  • To identify and characterize specific molecular markers for human prostate stem cells.
  • To investigate the potential of identified cells in reconstituting prostatic structures.

Main Methods:

  • Utilized immunophenotyping to identify cell surface markers on prostate basal cells.
  • Assessed the expression of the CD133 antigen (AC133) in conjunction with alpha(2)beta(1) integrin expression.

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  • Evaluated the in vitro proliferative potential of identified cell populations.
  • Grafted cells into immunocompromised male nude mice to assess their ability to form prostatic-like acini.

    • Main Results:

    • A distinct subpopulation of human prostate basal cells (approximately 1%) was identified expressing the CD133 antigen.
    • These CD133-positive cells were found to be restricted to the alpha(2)beta(1)(hi) population, a previously suggested marker for prostate stem cells.
    • The alpha(2)beta(1)(hi)/CD133(+) cells demonstrated significant in vitro proliferative capacity.
    • These cells were capable of reconstituting prostatic-like acini in vivo in immunocompromised male nude mice.

    Conclusions:

    • The alpha(2)beta(1)(hi)/CD133(+) cell population represents a promising candidate for human prostate epithelial stem cells.
    • These findings provide a crucial marker for isolating and studying prostate stem cells, advancing research into prostate cancer and benign prostatic hyperplasia.