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Updated: Aug 23, 2026

A Preterm Rat Model for Pain Studies
Published on: February 9, 2024
Effects of age, gender and holding on pain response during infant immunization
Moshe Ipp1, Anna Taddio, Morton Goldbach
1Department of Paediatrics, The Hospital for Sick Children, Toronto, Canada.
Insights
Infant positioning during immunization does not affect pain. However, younger infants (2 months) show greater pain responses than older infants (4-6 months).
Area of Science:
- Pediatrics
- Pain Management
- Immunization Practices
Background:
- Assessing infant pain is crucial for evaluating analgesic effectiveness.
- Routine immunizations are a common source of acute pain in infants.
- Understanding factors influencing infant pain can improve clinical practice.
Purpose of the Study:
- To investigate the impact of infant positioning (supine vs. held) on pain responses during routine immunizations.
- To examine the influence of infant age and gender on pain responses during immunization.
Main Methods:
- A randomized controlled trial involving 106 infants aged 2 to 6 months.
- Infants were randomized to either a supine (SUP) position or held (HLD) by a parent during immunization.
- Pain was assessed via duration of crying, facial grimacing, and visual analogue scale (VAS) scores.
Main Results:
- No significant differences in crying duration, facial grimacing, or VAS pain scores were found between the supine and held positions.
- Infant gender did not influence pain responses.
- Two-month-old infants exhibited significantly higher pain responses compared to 4- and 6-month-old infants.
Conclusions:
- Infant positioning (supine or held) does not alter pain responses during routine immunizations.
- Age is a significant factor, with younger infants experiencing more pain.
- These findings can inform strategies to minimize pain during pediatric immunizations.
Abstract:
Determinants of infant pain responses are important when assessing the efficacy of analgesics. In a randomized controlled trial, 106 infants aged 2 to 6 months were positioned either supine (SUP) on the examination table or held (HLD) by a parent during routine immunization in a community pediatric office. There was no difference between the SUP and HLD infants in duration of crying, facial grimacing or visual analogue scale (VAS) pain scores. Similarly gender did not affect pain response. In contrast, 2-month-old infants displayed more pain during immunization than did 4 or 6-month-old infants.
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