Related Experiment Video
Updated: Aug 23, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
Determination of acid/base dissociation constants based on a rapid detection of the half equivalence point by
Hideji Tanaka1, Takahiro Tachibana
1Faculty of Pharmaceutical Sciences, Tokushima University, Shomachi 1-78-1, Tokushima 770-8505, Japan. htanaka@ph.tokushima-u.ac.jp
Abstract:
Acid dissociation constants (Ka) were determined through the rapid detection of the half equivalence point (EP1/2) based on a feedback-based flow ratiometry. A titrand, delivered at a constant flow rate, was merged with a titrant, whose flow rate was varied in response to a control voltage (Vc) from a controller. Downstream, the pH of the mixed solution was monitored. Initially, Vc was increased linearly. At the instance that the detector sensed EP1/2, the ramp direction of Vc changed downward. When EP1/2 was sensed again, Vc was increased again. This series of process was repeated automatically. The pH at EP1/2 was regarded as being pKa of the analyte after an activity correction. Satisfactory results were obtained for different acids in various matrices with good precision (RSD approximately 3%) at a throughput rate of 56 s/determination.
Related Concept Videos
Titration of a Weak Acid with a Weak Base
As a result, there is no simple...
Titration of Polyprotic Base with a Strong Acid
Titration of a Weak Acid with a Strong Base
Acid–Base Titration: Overview
Acid-Base Titration Curves
For a titration carried out for 25.00 mL of 0.100...
Titration of Polyprotic Acids with a Strong Base

