Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput automated post-processing of separation data.

Jonathan G Shackman1, Christopher J Watson, Robert T Kennedy

  • 1Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA.

Journal of Chromatography. A
|July 3, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.

Analytical chemistry·2026
Same author

Integrating mass spectrometry with Nanopore direct RNA sequencing for <i>de novo</i> modification profiling of bacteriophage MS2.

bioRxiv : the preprint server for biology·2026
Same author

RNase 4 improves bottom-up modification mapping of <i>E. coli</i> total tRNAs using HILIC-MS/MS.

bioRxiv : the preprint server for biology·2026
Same author

Utilizing Venturi effect for automated high-throughput droplet-MS from well plates.

The Analyst·2026
Same author

Rapid analysis of terpenes produced by fermentation using flow injection analysis coupled to APCI MS.

Analytical methods : advancing methods and applications·2026
Same author

Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.

ACS measurement science au·2025
Same journal

Stereo-sensitive modelling of gas chromatographic retention indices of mono-cycloalkanes in jet fuel range.

Journal of chromatography. A·2026
Same journal

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same journal

MOF-supported surface-imprinted polymer for hazard governance of aristolochic acids in herbal matrices: A safety-control strategy supported by multiscale simulations.

Journal of chromatography. A·2026
Same journal

Portable cold-assisted head-space solid-phase microextraction coupled with GC-MS/MS for sensitive determination of trace polychlorinated naphthalenes in water.

Journal of chromatography. A·2026
Same journal

Characterization of phosphorous impurities originating from the synthesis of Sarin.

Journal of chromatography. A·2026
Same journal

Extraction and chromatographic purification of purpurin: A scalable approach using modified dry column vacuum chromatography.

Journal of chromatography. A·2026
See all related articles

A new computer program, "Cutter," offers efficient high-throughput analysis of electropherograms and chromatograms. It significantly reduces analysis time and accurately determines chromatographic figures of merit, including peak deconvolution.

Area of Science:

  • Analytical Chemistry
  • Computational Chemistry

Background:

  • High-throughput analysis of serial electropherograms/chromatograms is crucial for scientific research.
  • Existing methods can be time-consuming and lack efficiency for large datasets.

Purpose of the Study:

  • To develop an efficient computational method for high-throughput analysis of multiple electropherograms or chromatograms.
  • To introduce a software program, "Cutter," for automated determination of chromatographic figures of merit (CFOM).

Main Methods:

  • Batch processing of serial separations using the "Cutter" program.
  • Determination of CFOM including peak centroid times, heights, areas, signal-to-noise ratios (S/N), variance, skew, excess, and plate number.
  • Automated peak deconvolution utilizing an Empirically Transformed Gaussian function.

Related Experiment Videos

Main Results:

  • The "Cutter" software demonstrated accuracy comparable to or better than commercial software, with relative errors below 5% for area calculations at S/N = 5.
  • Analysis of 1300 electropherograms was completed in 5 minutes, a nearly 200-fold time reduction.
  • Deconvoluted peak area measurements showed high accuracy, within 3% of true values for well-resolved peaks and within 10% for overlapped peaks.

Conclusions:

  • The "Cutter" program provides an efficient and accurate solution for high-throughput analysis of serial chromatographic and electrophoretic data.
  • The novel automated peak deconvolution method enhances the reliability of quantitative analysis, even for complex mixtures.
  • This advancement significantly accelerates data processing in analytical chemistry applications.