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Hypochlorite scavenging activity of flavonoids
Omidreza Firuzi1, Premysl Mladenka, Rita Petrucci
1Dipartimento di Farmacologiadelle Sostanze Naturali e Fisiologia Generale, Universitá di Roma "La Sapienza", P. le Aldo Moro 5, 00185 Rome, Italy.
The Journal of Pharmacy and Pharmacology
|July 3, 2004
Summary
Flavonoids effectively neutralize hypochlorite, a harmful oxidant. This discovery suggests potential therapeutic uses for flavonoids in diseases linked to hypochlorite, such as atherosclerosis.
Area of Science:
- Biochemistry
- Pharmacology
Background:
- Hypochlorite is a reactive oxidant produced by activated phagocytes, implicated in various diseases.
- Flavonoids, known for antioxidant properties, have largely uncharacterized reactivity with hypochlorite.
Purpose of the Study:
- To investigate the hypochlorite scavenging activity of various flavonoids.
- To identify structural features of flavonoids that contribute to hypochlorite scavenging.
Main Methods:
- A microplate assay was used to evaluate the inhibition of chloramine formation in human serum albumin.
- Thirteen different flavonoids were tested for their hypochlorite scavenging capacity.
Main Results:
- Most tested flavonoids demonstrated significant hypochlorite scavenging activity at micromolar concentrations.
- Flavonoid activity correlated positively with the number of hydroxyl substitutions, especially at the 3-position.
- Activity was not dependent on a 2,3-double bond in the C ring.
Conclusions:
- Flavonoids are potent in vitro hypochlorite scavengers.
- Structural characteristics, particularly hydroxyl group presence and position, are crucial for scavenging efficacy.
- Flavonoids show promise for therapeutic applications in hypochlorite-mediated diseases like atherosclerosis.