Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Organization01:13

Protein Organization

Overview
Protein Organization01:13

Protein Organization

Overview
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure and function of IWS1 in transcription elongation.

Nucleic acids research·2026
Same author

In silico discovery of nanobody binders to a G-protein coupled receptor using AlphaFold-Multimer.

Nature communications·2026
Same author

Factors Influencing Post-Operative Oral Intake Following Tonsillectomy in Children with OSA.

The Annals of otology, rhinology, and laryngology·2026
Same author

Anti-Epstein-Barr virus (EBV) antibodies in EBV-associated gastric carcinoma.

Infectious agents and cancer·2026
Same author

The Use and Utility of Wearable Devices for Tracking Sleep and Activity in Inpatient Mental Health Settings: Protocol for a Rapid Review.

JMIR research protocols·2026
Same author

Understanding digital navigators in health care: a scoping review protocol.

JBI evidence synthesis·2026
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2026

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Self-assembling protein microarrays.

Niroshan Ramachandran1, Eugenie Hainsworth, Bhupinder Bhullar

  • 1Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 320 Charles Street, Cambridge, MA 02141, USA.

Science (New York, N.Y.)
|July 3, 2004
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel protein microarray technique using complementary DNA and in situ translation. This method simplifies protein production for functional studies, overcoming common challenges in protein microarray development and application.

More Related Videos

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

Related Experiment Videos

Last Updated: Jun 25, 2026

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Proteomics

Background:

  • Protein microarrays are valuable for studying protein function but face challenges in protein production.
  • Traditional methods require protein purification and can lead to stability issues, limiting widespread use.

Purpose of the Study:

  • To develop an improved method for generating protein microarrays.
  • To overcome limitations associated with protein production and stability for functional analysis.

Main Methods:

  • Generated protein microarrays by printing complementary DNA (cDNA) onto glass slides.
  • Utilized in situ translation with mammalian reticulocyte lysate to produce target proteins.
  • Employed epitope tags for in situ immobilization of translated proteins.

Main Results:

  • Successfully created functional protein microarrays without protein purification.
  • Demonstrated the utility of the method by mapping interactions among 29 human DNA replication proteins.
  • Recapitulated Cdt1 binding regulation and mapped its geminin-binding domain.

Conclusions:

  • The developed cDNA-based in situ translation method simplifies protein microarray production.
  • This approach enhances protein availability for functional studies and overcomes stability concerns.
  • The technology is effective for mapping protein interactions and regulatory mechanisms.