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Updated: Aug 5, 2026

Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
[The use of neck skin for microbial process control of fresh poultry meat using the bioluminescence method]
1Bundesinstitut für Risikobewertung, Berlin.
Abstract:
In this study comparative investigations of the total viable counts of bacteria and viable counts of Enterobacteriaceae with the ATP content were carried out on 70 poultry carcasses and neck skins thereof. The results revealed a correlation of total viable counts on the neck skin and on the carcass sample. Therefore the examination on total viable counts of neck skin is able to give results on the respective load of the carcass (coefficient of correlation (r) = 0.85 and coefficient of determination (R2) = 0.71). This only applies, however, to a slaughter technology at which the poultry hangs up site down with the neck skin to the bottom. The results of the ATP investigation expressed in Relative Light Units (RLU) in a bioluminescence method and total viable counts of neck skin samples are correlated (r = 0.85; R2 = 0.72). Lower values for r and R2 were estimated for RLU/Enterobacteriaceae on neck skin (r = 0.64; R2 = 0.41), for RLU/total viable counts on the carcass (r = 0.66; R2 = 0.46) and for RLU/Enterobacteriaceae on the carcass (r = 0.33; R2 = 0.11). To estimate the hygiene status in poultry slaughtery, sampling from the neck skin can replace the sampling on the whole carcass The bioluminescence method is suitable to replace the determination of total viable counts in the context of in-house hygiene supervision. However, this method seemed to be less reliable to predict the Enterobacteriaceae counts.
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