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Development of a quantitative high-performance liquid chromatography-photodiode array detection measurement system
Rebecca J Robbins1, Scott R Bean
1Food Composition Laboratory, Beltsville Human Nutrition Research Center, US Department of Agriculture, Building 161, BARC, East Beltsville, MD 20705-3000, USA. robbins@bhnrc.usda.gov
Journal of Chromatography. A
|July 6, 2004
Summary
A new method accurately quantifies 16 phenolic acids in wine using high-performance liquid chromatography. This validated technique ensures reliable results for wine analysis and quality control.
Area of Science:
- Analytical Chemistry
- Food Chemistry
Background:
- Phenolic acids are important compounds in wine, influencing its sensory properties and health benefits.
- Accurate quantification of phenolic acids is crucial for wine quality assessment and authenticity verification.
Purpose of the Study:
- To develop and validate a quantitative method for analyzing 16 phenolic acids in wine.
- To optimize sample preparation and chromatographic separation for accurate phenolic acid determination.
Main Methods:
- High-performance liquid chromatography with photodiode array detection (HPLC-PDA) was employed.
- A Phenomenex Luna C18 column with a methanol-water gradient containing formic acid was used for separation.
- Optimized extraction procedures yielded 79-87% recovery.
Main Results:
- The method successfully separated and quantified 16 individual phenolic acids.
- Inter-laboratory validation demonstrated high correlation (R2: 0.990-0.999) between datasets.
- Optimized extraction and HPLC-PDA conditions provided reliable quantitative results.
Conclusions:
- A robust and validated HPLC-PDA method for phenolic acid quantification in wine has been established.
- The method offers high accuracy and precision, suitable for routine wine analysis.
- This contributes to improved wine quality control and research into phenolic acid profiles.