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Updated: Aug 23, 2026

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A rapid and sensitive method for the detection of Xanthomonas fragariae, causal agent of angular leafspot disease in
Anna Stöger1, Werner Ruppitsch
1Austrian Agency for Health and Food Safety, Competence Center of Biochemistry, Spargelfeldstrasse 191, A-1226 Vienna, Austria.
Abstract:
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming, too insensitive or impractical when handling a large number of samples routinely. Here we describe a modified protocol of the REDExtract-N-Amp Plant PCR-Kit for the detection of X. fragariae in planta and demonstrate that it provides greater sensitivity, speed and high throughput potential than methods previously described.
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