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Related Experiment Videos

Extracellular H+ dynamics during oogenesis in Rhodnius prolixus ovarioles.

C S Bjornsson1, E Huebner

  • 1Laboratory of Nervous System Disorders, Wadsworth Center, Albany, NY 12201-0509, USA.

The Journal of Experimental Biology
|July 6, 2004
PubMed
Summary

Hydrogen ion (H+) fluxes were measured during insect oogenesis. These fluxes in the interfollicular stalk and follicle cells may regulate key developmental events like vitellogenesis and endocytosis.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Insect Physiology

Background:

  • Oogenesis involves complex cellular processes regulated by pH.
  • Hydrogen ion (H+) dynamics are crucial for cellular functions but poorly understood during oogenesis.
  • Rhodnius prolixus ovarioles exhibit significant polarity, suggesting specialized transport mechanisms.

Purpose of the Study:

  • To investigate the spatiotemporal dynamics of transmembrane hydrogen ion (H+) fluxes during oogenesis in Rhodnius prolixus.
  • To correlate H+ flux patterns with specific stages of oogenesis and follicle development.
  • To explore the potential roles of H+ fluxes in regulating oogenic events.

Main Methods:

  • Utilized a non-invasive, self-referencing, H+-selective probe to measure extracellular H+ fluxes.

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  • Monitored H+ dynamics along the entire length of Rhodnius ovarioles throughout an oogenesis cycle.
  • Correlated flux measurements with morphological observations of follicle development and ovulation.

    • Main Results:

    • Significant extracellular H+ efflux was detected in the interfollicular stalk, peaking during midvitellogenesis.
    • H+ efflux in the stalk preceded follicle stalk formation and the onset of vitellogenesis in adjacent follicles.
    • H+ efflux was also observed over the terminal follicle and around follicle cells post-ovulation, implicating the follicular epithelium.

    Conclusions:

    • Transmembrane H+ fluxes are dynamic and spatially regulated during Rhodnius oogenesis.
    • These fluxes likely play critical roles in regulating vitellogenesis onset, endocytosis, and follicle cell functions.
    • H+ transport may be involved in interfollicular communication and feedback mechanisms during development.