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Optisol corneal storage medium.

R L Lindstrom1, H E Kaufman, D L Skelnik

  • 1Department of Ophthalmology, University of Minnesota, Minneapolis.

American Journal of Ophthalmology
|September 15, 1992
PubMed
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Optisol, a new corneal storage medium, preserves endothelial cells for up to two weeks, offering flexibility for corneal transplants. It also results in thinner corneas, aiding surgical evaluation and manipulation.

Area of Science:

  • Ophthalmology
  • Corneal Transplantation
  • Tissue Preservation

Background:

  • Corneal storage media are crucial for maintaining donor tissue viability before transplantation.
  • Existing media have limitations in storage duration and tissue quality preservation.

Purpose of the Study:

  • To evaluate the safety and efficacy of Optisol, a novel corneal storage medium.
  • To compare Optisol with established media like McCarey-Kaufman, Dexsol, and K-Sol.

Main Methods:

  • In vitro studies using rabbit and human corneas to assess tissue thickness, endothelial cell mitotic activity (thymidine uptake), and cell morphology (specular microscopy, ultrastructural examination).
  • In vivo safety assessment in rabbit eyes using Optisol eye drops.
  • An open-label clinical study of penetrating keratoplasty patients receiving donor corneas stored in Optisol.

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Main Results:

  • In vitro: Optisol-stored corneas were thinner and showed better endothelial cell integrity and mitotic activity compared to Dexsol.
  • In vivo: Optisol eye drops demonstrated no epithelial toxicity or intraocular inflammation.
  • Clinical study: High graft clarity rates (93% at 3 months, 98% at 6 months) and acceptable endothelial cell loss (5.0% at 3 months, 11.5% at 6 months) were observed.

Conclusions:

  • Optisol effectively preserves corneal endothelial cells for up to two weeks at 4°C.
  • Optisol offers flexibility in donor tissue utilization for corneal transplantation.
  • Optisol-treated corneas are thinner, potentially improving surgical outcomes.