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Related Experiment Videos

Protamine assembled in multilayers on colloidal particles can be exchanged and released.

Sabine Hiller1, Stefano Leporatti, Andreas Schnäckel

  • 1Institute of Medical Physics and Biophysics, University of Leipzig, D-04103 Leipzig, Germany. reichls@medizin.uni-leipzig.de

Biomacromolecules
|July 13, 2004
PubMed
Summary

Protamine (PRM) multilayers on silica particles show significant PRM exchange, enabling smart drug release. Fluorescence activated cell sorting (FACS) effectively quantified particle aggregation during biocomposite film formation.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Layer-by-layer adsorption of biocomposite thin films on colloidal particles offers potential for drug delivery and diagnostics.
  • Protamine (PRM) and dextran sulfate (DXS) or bovine serum albumin (BSA) multilayers were explored for their assembly and properties.

Purpose of the Study:

  • To investigate the formation and stability of PRM/DXS and PRM/BSA multilayers on colloidal silica.
  • To assess the exchangeability of adsorbed PRM within the multilayers.
  • To evaluate particle aggregation during the coating process using fluorescence activated cell sorting (FACS).

Main Methods:

  • Fabrication of multilayers on colloidal silica using layer-by-layer adsorption.
  • Investigation using fluorescence activated cell sorting (FACS) and microelectrophoresis.

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  • Incorporation of fluorescein-labeled polyelectrolytes to monitor layer growth.

    • Main Results:

    • PRM/DXS multilayers demonstrated regular growth and stability, with up to 90% of adsorbed PRM available for exchange.
    • PRM/BSA did not form stable multilayers under tested conditions, despite zeta-potential alternations.
    • FACS effectively quantified particle aggregation, with PRM/DXS coated particles showing higher aggregation (approx. 50%) compared to PAH/PSS coated particles (approx. 10%).

    Conclusions:

    • The exchange capability of PRM in biocomposite multilayers suggests potential for developing smart drug release systems.
    • FACS is a suitable method for quantifying aggregation in nanoparticle coating processes.
    • The choice of materials significantly influences multilayer stability and particle aggregation behavior.