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Updated: Apr 6, 2026
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Author Spotlight: Developing a Unique Modular Microphysiological System to Mimic Human Barrier Tissue
Published on: February 16, 2024
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Tuneable Permeability of Cellulose Nanofibrils-based Membranes in Next-Generation Barrier-On-Chip Systems
Vita Guarino1,2,3, Johan Erlandsson4, Elisa De Luca2,3,5
1University of Salento, Department of Experimental Medicine, c/o Campus Ecotekne, Lecce, Italy.
Chembiochem : a European Journal of Chemical Biology
|April 4, 2026
Summary
Researchers developed novel cellulose nanofibril (CNF) membranes for in vitro barrier models. These biodegradable, natural membranes mimic human tissue and allow tunable permeability for advanced organ-on-chip systems.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cellular Biology
Background:
- Human body barriers regulate substance exchange, with altered permeability in disease.
- In vitro barrier models are vital for studying molecular diffusion.
- Current models often use artificial, non-biodegradable membranes.
Purpose of the Study:
- To introduce cellulose nanofibril (CNF)-based membranes for in vitro barrier systems.
- To develop a new class of biomimetic membranes for organ-on-chip (OoC) applications.
- To create more physiologically relevant in vitro models.
Main Methods:
- Developed CNF-based membranes from natural sources.
- Integrated CNF membranes into dual-chamber polydimethylsiloxane devices.
- Modulated membrane permeability via chemical and enzymatic treatments.
Main Results:
- CNF membranes exhibit nontoxicity, biodegradability, and optical transparency.
- The 3D fibrillar structure mimics the cellular basement membrane.
- Permeability was successfully modulated while maintaining cell adhesion and growth.
Conclusions:
- CNF-based membranes offer a promising alternative to artificial materials for in vitro barrier models.
- This technology can enhance next-generation OoC devices.
- CNF membranes provide more realistic mimicry of physiological human barriers.
