Quantitative analysis of sinistrin in serum with high-performance liquid chromatography for renal function testing

Area of Science:

• Analytical Chemistry
• Clinical Chemistry
• Biochemistry

Background:

• Sinistrin is a key biomarker for evaluating renal function.
• Accurate and efficient methods for sinistrin quantification are crucial for clinical diagnostics.
• Existing methods may require complex sample preparation or lack sensitivity.

Purpose of the Study:

• To develop a reliable and simple high-performance liquid chromatography (HPLC) method for quantitative sinistrin determination.
• To validate the method for use in serum and plasma samples.
• To assess the method's sensitivity and accuracy for renal function testing.

Main Methods:

• Development of an HPLC method with electrochemical detection for sinistrin measurement.
• Sample preparation involving enzymatic glucose removal, deproteinization, and acid hydrolysis.
• Separation of fructose, a hydrolysis product of sinistrin, via HPLC.

Main Results:

• The method demonstrates high recovery (near 100%) and a linear range up to 500 mg/L.
• Results correlate well with established enzymatic quantification methods.
• The assay achieves high sensitivity, measuring sinistrin down to 2 mg/L in aqueous solutions with good precision.

Conclusions:

• The developed HPLC method offers a sensitive, accurate, and straightforward approach for sinistrin quantification in biological fluids.
• This laboratory method is suitable for renal function testing and pharmacokinetic studies.
• The technique facilitates precise measurements, particularly at low sinistrin concentrations and with small sample volumes.