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Related Experiment Video

Updated: Jul 7, 2026

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
12:43

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Published on: December 6, 2013

Functional antigen-presenting leucocytes derived from human embryonic stem cells in vitro.

Xiangcan Zhan1, Gautam Dravid, Zhaohui Ye

  • 1Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Lancet (London, England)
|July 13, 2004
PubMed
Summary

Human embryonic stem (hES) cells can be differentiated into immune-modulating leucocytes. These hES-cell-derived antigen-presenting cells can elicit T-cell responses and may improve transplant acceptance.

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Last Updated: Jul 7, 2026

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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

Area of Science:

  • Stem cell biology
  • Immunology
  • Transplantation medicine

Background:

  • Human embryonic stem (hES) cells offer potential for cell transplantation therapies.
  • Graft rejection due to polymorphic MHC molecules on hES cells necessitates immune tolerance strategies.
  • Inducing donor-specific immune tolerance via chimeric engraftment is a key strategy for sustained engraftment.

Purpose of the Study:

  • To develop efficient methods for differentiating hES cells into hematopoietic cells, including immune-modulating leucocytes.
  • To generate antigen-presenting cells from hES cells for transplantation therapies.
  • To overcome graft rejection in allogeneic hosts without broad immunosuppression.

Main Methods:

  • Generation of hematopoietic cells from hES-generated embryonic bodies without murine stromal feeder cells.
  • Culture of embryonic bodies with hematopoietic cytokines.
  • Analysis of hES-derived hematopoietic cells using flow cytometry, colony-forming assays, cytochemical staining, and mixed leucocyte reactions.

Main Results:

  • Leukocytes expressing CD45 were generated and released for 6-7 weeks.
  • Erythroid and myeloid progenitor cells were produced.
  • Approximately 25% of generated leucocytes expressed MHC class II and costimulatory molecules, functioning as antigen-presenting cells.

Conclusions:

  • hES cell-derived antigen-presenting cells, resembling dendritic cells and macrophages, can elicit allogeneic T-cell responses.
  • These cells can be used to regulate alloreactive T cells.
  • The findings suggest potential for improving transplant acceptance of hES-cell derivatives through immune tolerance induction.