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Digital transcriptome analysis in the aging cerebellum
Magdalena C Popesco1, Adrienne Frostholm, Katarzyna Rejniak
1Department of Pharmacology and the Mathematical Biosciences Institute, The Ohio State University, Columbus, Ohio 43210, USA.
Annals of the New York Academy of Sciences
|July 13, 2004
Summary
Serial analysis of gene expression (SAGE) identified age-related changes in the mouse cerebellum transcriptome. Key genes like growth hormone and prolactin decreased in aged mice, suggesting roles in the aging process.
Area of Science:
- Neuroscience
- Genomics
- Molecular Biology
Background:
- The cerebellum's gene expression changes with age are not fully understood.
- Aging impacts various brain functions, necessitating molecular investigation.
Purpose of the Study:
- To comprehensively profile the adult and aged mouse cerebellum transcriptome using SAGE.
- To identify age-differentially expressed genes and novel transcripts in the cerebellum.
Main Methods:
- Serial analysis of gene expression (SAGE) was performed on adult (P92) and aged (P810) C57BL/6J mouse cerebella.
- A closest-neighbor algorithm filtered sequencing errors, and unique tags were categorized.
- Gene ontology classification (MmSAGEClass) was used for known genes.
Main Results:
- Identified and quantified expressed genes in adult and aged mouse cerebella.
- Observed significant downregulation of growth hormone and prolactin in aged cerebella.
- Discovered novel transcripts not present in the Unigene database, indicating new gene discoveries.
Conclusions:
- SAGE provides a detailed view of the cerebellar transcriptome across aging.
- Downregulation of specific genes suggests molecular mechanisms underlying cerebellar aging.
- The identified novel genes may represent new candidates for aging-related research.
