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Published on: March 30, 2019
[Inhibitory effect of MUC2 antisense oligodeoxynucleotide with lipofectin on human gastric cancer cell proliferation]
1Department of Pathology, Chongqing Medical University, Chongqing, 400016, P.R.China. y01029@163.com
Background & Objective:
Our previous study showed that the expression of MUC2 protein was related with the biological behavior of gastric carcinoma. The aim of the present study was to investigate the inhibitory effect in vitro of mucin gene MUC2 antisense oligodeoxynucleotide (ASODN) on its gene expression and cell proliferation on gastric cancer cells SGC7901.
Methods:
Phosphorothioate MUC2 ASODN was synthesized and transfected to SGC7901 cells mediated by lipofectin. Its inhibitory effects on cell proliferation was determined by MTT method, light and electron microscopy and immunohistochemical method.
Results:
The determination by MTT method demonstrated that MUC2 ASODN of varied concentration significantly inhibited the growth of SGC7901 cells while the control lipofectin and control N-ODN showed no such effect. The inhibitory effect was dose-dependent and time-dependent. The inhibition peaked at 48th hour after transfection, and the inhibition rate reached 55% when the MUC2 ASODN concentration was 0.5 micromol/L. After transfecting with MUC2 ASODN, SGC7901 cells showed decrease in number, volume, and karyokinesis, and increase in necroses under light microscopy. Mitochondrion swelling, increased liposomes, myelin figures, chromatin margination were found under electron microscopy. And the test by immunohistochemical method indicated that transfected MUC2 ASODN downregulated the expression levels of MUC2 protein, but upregulated the expression levels of p16 protein.
Conclusion:
MUC2 ASODN transfection could specifically inhibit SGC7901 cells proliferation.
Insights
Mucin gene MUC2 antisense oligodeoxynucleotide (ASODN) effectively inhibited gastric cancer cell proliferation in vitro. This targeted approach reduced MUC2 protein expression and suppressed tumor cell growth.
Area of Science:
- Molecular Biology
- Cancer Research
- Biochemistry
Context:
- Gastric carcinoma progression is linked to MUC2 protein expression.
- Targeting MUC2 offers a potential therapeutic strategy for gastric cancer.
Purpose:
- To investigate the in vitro inhibitory effects of MUC2 antisense oligodeoxynucleotide (ASODN) on gastric cancer cells (SGC7901).
- To assess the impact of MUC2 ASODN on MUC2 gene expression and cell proliferation.
Summary:
- MUC2 ASODN significantly inhibited SGC7901 cell proliferation in a dose- and time-dependent manner, with maximal inhibition at 48 hours.
- Morphological changes observed included decreased cell number and volume, increased necrosis, and alterations in mitochondrial structure.
- Immunohistochemical analysis confirmed MUC2 ASODN downregulated MUC2 protein and upregulated p16 protein expression.
Impact:
- MUC2 ASODN demonstrates specific inhibitory effects on gastric cancer cell proliferation.
- This study provides a basis for developing MUC2-targeted therapies for gastric carcinoma.