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Ectopic expression of DICE1 suppresses tumor cell growth

Ilse Wieland1, Christian Sell, Ulrich H Weidle

  • 1Institute of Human Genetics, Otto-von-Guericke-Universität, Magdeburg, Germany. ilse.wieland@medizin.uni-magdeburg.de

Oncology Reports
|July 16, 2004
PubMed

Insights

The tumor suppressor gene DICE1 inhibits cancer cell growth and colony formation. This gene

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The DICE1 tumor suppressor gene is located on chromosome 13q14.3, a region frequently associated with loss of heterozygosity in various cancers.
  • Down-regulation of DICE1 expression is observed in non-small cell lung carcinomas, suggesting its role in tumorigenesis.

Purpose of the Study:

  • To investigate the tumor-suppressive function of the DICE1 gene.
  • To determine DICE1's effect on the growth and anchorage-independent proliferation of carcinoma cell lines.

Main Methods:

  • Ectopic expression of DICE1 using DICE1-green fluorescent protein fusion constructs.
  • Assessing colony formation in human non-small cell lung carcinoma (SK-MES-1, NCI-H520) and prostate carcinoma (DU145) cell lines.
  • Evaluating growth suppression in IGF-IR transformed Balb/c 3T3 cells in soft agar.

Main Results:

  • Ectopic DICE1 expression inhibited colony formation in SK-MES-1, NCI-H520, and DU145 cell lines.
  • DICE1 significantly suppressed anchorage-independent growth of IGF-IR transformed Balb/c 3T3 cells.
  • These findings indicate DICE1 possesses growth-suppressing activity.

Conclusions:

  • DICE1 functions as a tumor suppressor by inhibiting cell proliferation and anchorage-independent growth.
  • DICE1 interferes with IGF-I signaling pathways crucial for the growth of transformed tumor cells.

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