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Stimulation of fetal spleen cells with lipopolysaccharides
LPS proved to be good B-cell mitogen for fetal pig spleen cells and induced proliferation as well as differentiation of virgin resting small lymphocytes within 48 hours of cultivation. The LPS-activated lymphocytes and blast-like cells were more intensely labeled with anti-Ig antibodies than control cells, most probably due to an increased density of surface Ig. A lower percentage of LPS-activated cells was found to be labeled with F(ab)2 fragment of anti-L than with anti-L antibodies having an intact Fc fragment. The latter finding suggests non-specific binding of antisera to Fc receptors which appear to be activated on LPS-stimulated lymphoid cells.
LPS proved to be good B-cell mitogen for fetal pig spleen cells and induced proliferation as well as differentiation of virgin resting small lymphocytes within 48 hours of cultivation. The LPS-activated lymphocytes and blast-like cells were more intensely labeled with anti-Ig antibodies than control cells, most probably due to an increased density of surface Ig. A lower percentage of LPS-activated cells was found to be labeled with F(ab)2 fragment of anti-L than with anti-L antibodies having an intact Fc fragment. The latter finding suggests non-specific binding of antisera to Fc receptors which appear to be activated on LPS-stimulated lymphoid cells.