Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

NDP kinase moves into developing primary cilia.

Kimberly A P Mitchell1, Betty C Gallagher, Gabor Szabo

  • 1Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, VA 22908-0736, USA.

Cell Motility and the Cytoskeleton
|July 20, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound as a noninvasive diagnostic tool to detect deep endometriosis using the International Deep Endometriosis Analysis terminology.

Fertility and sterility·2025
Same author

Demonstration of particulate matter characterization from HVO-blended diesel using an integrated multi-instrument approach.

Scientific reports·2025
Same author

The sodium-glucose cotransporter-2 inhibitor canagliflozin alleviates endothelial dysfunction in a rat bypass model.

Biochemical pharmacology·2025
Same author

Photoacoustic-Driven Retrieval of Complex Refractive Indices for Absorbing Aerosols.

Analytical chemistry·2025
Same author

Age, sex, and vessel region affect the vasomotor function and gene expression signature of the aorta in mice.

Journal of molecular and cellular cardiology plus·2025
Same author

Design principles of spacer cations for suppressing phase segregation in 2D halide perovskites.

Chemical science·2025
Same journal

Mechanics and dynamics of the cytoskeleton. Proceedings of a FEBS workshop. June 22-26, 2008. Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/ FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Mechanics and dynamics of the cytoskeleton: a special issue stemming from the 2008 ECF Meeting/FEBS Workshop (Mechanics and Dynamics of the Cytoskeleton) in Potsdam, Germany.

Cell motility and the cytoskeleton·2009
Same journal

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture.

Cell motility and the cytoskeleton·2009
Same journal

Centriole symmetry: a big tale from small organisms.

Cell motility and the cytoskeleton·2009
Same journal

Fluorescence-based quantitative scratch wound healing assay demonstrating the role of MAPKAPK-2/3 in fibroblast migration.

Cell motility and the cytoskeleton·2009
See all related articles

Nucleoside diphosphate (NDP) kinases A and B are found in primary cilia of cells, suggesting a universal role. These enzymes are active within cilia and may regulate their growth and function.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Primary cilia are sensory organelles involved in various cellular functions.
  • Nucleoside diphosphate (NDP) kinases are enzymes crucial for cellular energy metabolism.

Purpose of the Study:

  • To investigate the localization and function of NDP kinases within primary cilia.
  • To determine if NDP kinases play a role in primary cilia structure and signaling.

Main Methods:

  • Immunofluorescence staining of NIH3T3 fibroblasts and Xenopus A6 kidney epithelial cells.
  • Isolation of primary cilia-enriched fractions for biochemical analysis.
  • Enzyme activity assays and subciliary localization studies.

Main Results:

Related Experiment Videos

  • NDP kinases A and B localize to primary cilia in both murine and Xenopus cells.
  • Translocation into cilia is size-dependent, occurring at a critical length of 5-6 microm.
  • Ciliary NDP kinase is enzymatically active and associated with the ciliary membrane and matrix, not the axoneme.

Conclusions:

  • NDP kinases are universal components of primary cilia, with a distinct subciliary distribution.
  • The translocation and activity suggest NDP kinases regulate primary cilia elongation and maintenance.
  • NDP kinases may regenerate GTP for microtubule turnover and transmembrane signaling in cilia.