Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Discs large (Dlg1) complexes in lymphocyte activation.

Ramnik Xavier1, Shahrooz Rabizadeh, Kazuhiro Ishiguro

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

The Journal of Cell Biology
|July 21, 2004
PubMed
Summary

Discs large (Dlg1) protein is recruited to T cell activation sites. Dlg1 regulates T cell receptor signaling, with its reduction enhancing activation, suggesting a role in attenuating immune responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Comparing Resident Team Performance in Complex Surgical Oncology: A Single-Institution Cohort Study.

Annals of surgical oncology·2026
Same author

Non-interruptive decision support to increase appropriate screening for primary hyperparathyroidism.

American journal of surgery·2026
Same author

Genetic Susceptibility to Incisional Hernia Evaluation of Hernia Polygenic Risk Scores.

medRxiv : the preprint server for health sciences·2026
Same author

Half a century of pneumococcal vaccination in sickle cell disease: Are we winning the battle or losing immunity?

Vaccine·2026
Same author

Comparing Resident Team Performance in Complex Surgical Oncology: A Single-Institution Cohort Study.

Annals of surgical oncology·2026
Same author

Patient Outcome Reports for Senior Residents: An Opportunity to Foster Reflection and Identify Outliers for Intervention.

Journal of surgical education·2026

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • T cell activation relies on a structured interface for signal transmission.
  • The molecular mechanisms organizing T cell signaling machinery are not fully understood.

Purpose of the Study:

  • To investigate the role of Discs large (Dlg1) protein in T cell activation.
  • To elucidate the function of Dlg1 in regulating T cell receptor signaling pathways.

Main Methods:

  • Recruitment of Dlg1 to cortical actin upon T cell activation.
  • Analysis of Dlg1 complex formation with early signaling molecules.
  • Overexpression and RNA interference studies to assess Dlg1 function.
  • Measurement of NFAT reporter activation and T cell-mediated signaling.

Related Experiment Videos

Main Results:

  • Dlg1 is recruited to cortical actin during T cell activation and forms complexes with signaling molecules.
  • Dlg1 overexpression attenuates basal and Vav1-induced NFAT reporter activation.
  • Reduced Dlg1 expression enhances CD3- and superantigen-mediated NFAT activation.
  • Antigen receptor signaling attenuation involves segregation of early signaling complex elements.

Conclusions:

  • Dlg1 plays a critical role in regulating T cell activation.
  • Dlg1 functions to attenuate T cell receptor signaling.
  • The findings highlight the complex, orchestrated nature of T cell signaling attenuation.