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Related Experiment Videos

Laboratory scale structural genomics.

Brent W Segelke1, Johana Schafer, Matthew A Coleman

  • 1Macromolecular Crystallography and Structural Genomics Group, Biology and Biotechnology Research Program, P.O. Box 808, Lawrence Livermore National Laboratory, Livermore, CA 94551, USA.

Journal of Structural and Functional Genomics
|July 21, 2004
PubMed
Summary
This summary is machine-generated.

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Lawrence Livermore National Laboratory developed a structural genomics crystallization facility, integrating proteomics research. This facility streamlines protein structure determination using innovative methods and automation for academic labs.

Area of Science:

  • Structural biology
  • Proteomics
  • Genomics

Background:

  • The development of the Tuberculosis (TB) structural genomics consortium crystallization facility at Lawrence Livermore National Laboratory.
  • Parallel growth of local proteomics research efforts stemming from gene expression microarray and comparative genomics studies.
  • Leveraging collective experience from TB consortium labs and the NIH-NIGMS protein structure initiative.

Purpose of the Study:

  • To explore the possibilities and challenges of structural genomics on an academic laboratory scale.
  • To detail procedures and protocols for a comprehensive structural genomics workflow.
  • To demonstrate the feasibility of a small-group, cost-effective approach to structural genomics.

Main Methods:

  • Genomic targeting approaches and primer design.

Related Experiment Videos

  • Cloning, small-scale expression screening, and scale-up/purification.
  • Automated crystallization screening and data collection using a combination of traditional and innovative molecular biochemistry approaches, software automation, and robotic equipment.

    • Main Results:

    • Establishment of a functional structural genomics crystallization facility.
    • Development of efficient protocols for protein expression, purification, and crystallization.
    • Successful implementation of automated screening and data collection.

    Conclusions:

    • Structural genomics is achievable on an academic laboratory scale.
    • A combination of traditional methods, innovative biochemistry, automation, and robotics can facilitate structural genomics.
    • The described protocols provide a framework for other academic labs to pursue structural genomics.