Neutral density liquid formulations for nematode-based biopesticides

Area of Science:

• Agricultural Science
• Nematology
• Biological Pest Control

Background:

• Entomopathogenic nematodes are crucial biological insecticides for managing agricultural and horticultural pests.
• Current commercial formulations often use dehydrated nematodes on solid carriers, leading to poor survival, handling difficulties, and species-specific limitations.

Purpose of the Study:

• To develop an improved liquid formulation for the storage and transport of entomopathogenic nematodes.
• To enhance nematode survival and virulence compared to existing methods.

Main Methods:

• A novel, non-viscous, non-adhesive, and non-toxic liquid formulation based on neutral density colloidal silica suspensions was developed.
• Nematode survival and virulence were assessed after storage in the colloidal silica formulation without aeration.
• Comparison was made with nematodes stored in aerated quarter strength Ringer's solution.

Main Results:

• Nematodes stored in the colloidal silica liquid formulation exhibited superior survival rates.
• Virulence of nematodes stored in the new formulation was also significantly higher.
• The formulation allowed for storage without aeration, simplifying practical application.

Conclusions:

• The neutral density colloidal silica suspension provides an effective and stable liquid formulation for entomopathogenic nematodes.
• This innovative approach overcomes the limitations of dehydration-based methods, improving nematode viability and efficacy as biological insecticides.
• The formulation holds promise for broader application and improved delivery of nematode-based pest control strategies.