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[Comparison of sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm
Meiyi Liu1, Shunmei Deng, Chunjie Ma
1Human Semen Bank, Guangdong Family Planning Hospital, Guangzhou, Guangdong 510600, China. dxlmyi@pub.guangzhou.gd.cn
Objective:
To observe sperm motion parameters in pre-freeze and post-thaw semen samples using computer-assisted sperm analysis (CASA) system.
Methods:
Semen analyses of 238 samples before freezing and after thawing were separately performed by Hamilton-Thorne Sperm Analyzer.
Results:
Sperm motility in post-thaw samples was significantly decreased. There was significant correlation and difference between pre-freeze and post-thaw samples in sperm motion parameters, including average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), straightness (STR) and linearity (LIN), except heat cross frequency (BCF). The percentage of sperm movement velocity parameters (VAP, VSL and VCL) and moving pattern parameters (ALH) significantly decreased, while that of LIN and STR significantly increased in post-thaw samples.
Conclusion:
CASA system is of clinically applied value and is a useful tool for evaluating sperm motion parameters in pre-freeze and post-thaw semen samples.