Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ocular glucose extraction using vitreoperfusion in the cat.

N P Blair1, J J Moy, Y Tsukahara

  • 1Department of Ophthalmology and Visual Sciences, UIC Eye Center, University of Illinois, Chicago College of Medicine.

Investigative Ophthalmology & Visual Science
|September 1, 1992
PubMed
Summary

Researchers developed a mathematical model to measure ocular glucose consumption in vivo, finding that eyes retain glucose extraction ability for at least 4 hours during ischemia. Glucose extraction rates increased over time, suggesting potential for future diagnostic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell death signalling is competitively but coordinately regulated by repressor-type and activator-type ethylene response factors in tobacco (Nicotiana tabacum) plants.

Plant biology (Stuttgart, Germany)·2022
Same author

Autoimmune pulmonary alveolar proteinosis developed during immunosuppressive treatment in polymyositis with interstitial lung disease: a case report.

BMC pulmonary medicine·2020
Same author

Across and within breed differences in the relationship between packed cell volume and fecal egg count in growing meat goat and hair sheep males naturally and artificially infected with gastrointestinal nematodes.

Veterinary parasitology, regional studies and reports·2019
Same author

Effect of Ovar-DRA and Ovar-DRB1 genotype in small ruminants with haemonchosis.

Parasite immunology·2018
Same author

EVALUATION OF PATIENT EXPOSURE IN FAST kVp SWITCHING DUAL ENERGY COMPUTED TOMOGRAPHY: PHANTOM STUDY.

Radiation protection dosimetry·2018
Same author

Effects of level of brackish water on feed intake, digestion, heat energy, and blood constituents of growing Boer and Spanish goat wethers.

Journal of animal science·2016

Area of Science:

  • Ophthalmology
  • Metabolic Research
  • Mathematical Modeling

Background:

  • Ocular glucose consumption, primarily by the retina, offers insights into eye health and disease.
  • Vitreoperfusion can preserve retinal tissue during ischemia by providing the sole extraretinal glucose source.

Purpose of the Study:

  • To develop a mathematical model for quantifying in vivo ocular glucose consumption (Eglu).
  • To assess the eye's ability to extract glucose from vitreoperfusion fluid during induced ischemia.

Main Methods:

  • A mathematical model was created to estimate glucose extraction rate (Eglu), accounting for metabolic uptake and outflow.
  • Model validation was performed using a glass eye model and fixed cat eyes.
  • In vivo experiments involved inducing ischemia in cat eyes with vitreoperfusion, followed by measurements of glucose concentration over time.

Related Experiment Videos

Main Results:

  • The mathematical model accurately predicted glucose decline in control models (glass eye: r=0.97; fixed cat eyes: r=0.96).
  • In ischemic cat eyes, mean Eglu values increased significantly over the observation period (15-255 min).
  • The eyes demonstrated sustained glucose extraction capability for up to 4 hours of ischemia.

Conclusions:

  • The developed model effectively quantifies ocular glucose consumption.
  • Eyes maintain glucose extraction ability during prolonged ischemia, though the rate increases over time.
  • Ocular glucose consumption measurements (Eglu) show promise for evaluating eye physiology and pathology.