Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A complete genetic analysis of neuronal Rab3 function.

Oliver M Schlüter1, Frank Schmitz, Reinhard Jahn

  • 1Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9111, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|July 23, 2004
PubMed
Summary

Mice lacking all four Rab3 proteins (Rab3A, Rab3B, Rab3C, Rab3D) die shortly after birth due to respiratory failure, indicating Rab3

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Psilocybin-induced behavioral and cellular effects.

Journal of neurogenetics·2026
Same author

Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq.

Cell reports methods·2026
Same author

Astrocytic D1 Dopamine-Signaling Regulates Synaptic Remodeling and Cocaine Seeking.

bioRxiv : the preprint server for biology·2026
Same author

Identity crisis: exploring the boundaries of cell type identification in the age of single-cell transcriptomics.

Frontiers in cellular neuroscience·2026
Same author

SNAP47 Silencing Impairs the Morphology and Neurotransmission of Hippocampal GABAergic Neurons.

Molecular neurobiology·2026
Same author

Assessing VirScan serosurvey epitope profiling variability between in-clinic venous blood draw and capillary blood self-sampling device.

Microbiology spectrum·2026

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Rab3 proteins (Rab3A-D) are GTP-binding proteins found in synaptic vesicles.
  • They are implicated in the process of neurotransmitter release.
  • The specific roles and redundancies of these Rab3 isoforms were not fully understood.

Purpose of the Study:

  • To investigate the in vivo functions of Rab3 proteins.
  • To determine the necessity of Rab3 proteins for mouse survival and neurotransmitter release.
  • To explore the functional redundancy among Rab3A, Rab3B, Rab3C, and Rab3D.

Main Methods:

  • Generation of single, double, triple, and quadruple Rab3 knock-out (KO) mouse models.
  • Viability and fertility assessments of KO mice.

Related Experiment Videos

  • Analysis of synapse structure and brain composition.
  • Electrophysiological studies on cultured hippocampal neurons from quadruple KO mice.

    • Main Results:

    • Single and double Rab3 KO mice are viable and fertile.
    • Triple KO mice lacking Rab3A mostly perish, while those expressing Rab3A are viable.
    • Quadruple KO mice die postnatally from respiratory failure, showing reduced evoked neurotransmitter release due to decreased release probability.
    • Loss of rabphilin, a Rab3-binding protein, was observed in Rab3-deficient mice.

    Conclusions:

    • Rab3 proteins are essential for mouse survival, with functional redundancy among the four isoforms.
    • Rab3 proteins are crucial modulators of Ca2+-triggered synaptic vesicle exocytosis, impacting release probability.
    • Rab3 is not essential for basic synaptic membrane traffic but regulates the core release machinery.